AccuBlotTM anti-Mouse IgG-HRP
Croyez AccuBlotTM anti-Mouse IgG-HRP is conjugated with high-activity HRP.
In co-immunoprecipitation (co-IP) assay, denatured IgG from IP samples may
shows interference result in immunoblotting at ~55 kDa (heavy chain) and ~25
kDa (light chain) by using traditional secondary antibodies. The products of
AccuBlotTM series are suitable in native IgG detection.
In co-immunoprecipitation (co-IP) assay, denatured IgG from IP samples may
shows interference result in immunoblotting at ~55 kDa (heavy chain) and ~25
kDa (light chain) by using traditional secondary antibodies. The products of
AccuBlotTM series are suitable in native IgG detection.
Product type:
Monoclonal
Immunogen:
Native mouse IgG
Host:
Rat
Reactivity:
Native mouse IgG2b
Conjugation:
HRP (RZ>3)
Form:
Liquid
Storage Buffer:
Phosphate Buffered Saline containing 1% BSA, 0.01% thimerosal, 50% glycerol, pH 7.4.
Storage:
Protect from light.
Store at 4°C for at least 6 months.
Store at -20°C for long-term preservation. Avoid repeated freeze/thaw cycles.
Application:
ELISA, WB
Manual:
1:1000-1:3000 for WB
1:2500-1:10000 for ELISA
Working dilution for specific application should be determined by the investigator to obtain the best conditions.
Shipping Conditions:
Blue ice
Monoclonal
Immunogen:
Native mouse IgG
Host:
Rat
Reactivity:
Native mouse IgG2b
Conjugation:
HRP (RZ>3)
Form:
Liquid
Storage Buffer:
Phosphate Buffered Saline containing 1% BSA, 0.01% thimerosal, 50% glycerol, pH 7.4.
Storage:
Protect from light.
Store at 4°C for at least 6 months.
Store at -20°C for long-term preservation. Avoid repeated freeze/thaw cycles.
Application:
ELISA, WB
Manual:
1:1000-1:3000 for WB
1:2500-1:10000 for ELISA
Working dilution for specific application should be determined by the investigator to obtain the best conditions.
Shipping Conditions:
Blue ice